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Library preparation |
Blocking |
Target capture |
Exome sequencing |
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To learn more, visit Whole Exome Sequencing solution |
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Solid tumor analysis |
NanOnCT Panel |
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Hematologic malignancy analysis |
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Low-frequency mutation analysis |
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To learn more, visit Low-frequency Mutation Analysis solution |
Fig 1. Capture performance of exome sequencing. The gDNA and FFPE libraries were prepared using NadPrep library kits for MGI. Exome Plus Panel v2.0 was used for target enrichment. The enriched libraries were sequenced on DNBSEQ-G400 (PE150) and subsampled to 35 M read pairs for the analysis of A. mappablity, B. on-target rate and C. coverage uniformity.
Table 1. Performance of the NanOnCT Panel v1.0
|
Mappability (%) |
On-target rate (%) |
Duplication (%) |
gDNA-1 |
99.75 |
87.32 |
2.91 |
gDNA-2 |
99.81 |
85.28 |
2.91 |