NanoRV Panel v1.0-Tech Access targets 36 species respiratory virus from 12 genera in 7 families. Both the reference genomic sequences and “neighbor” viral genomes have been taken into consideration in the panel design.
Table 1. Human respiratory virus targeted by NanoRV Panel v1.0-Tech Access
Family |
Genus |
Species covered |
Coronaviridae |
Alphacoronavirus ,Betacoronavirus |
2 alpha and 13 beta |
Orthomyxoviridae |
Alphainfluenzavirus, Betainfluenzavirus, Gammainfluenzavirus |
Influenza A virus, Influenza B virus, and Influenza C virus |
Paramyxoviridae |
Respirovirus,Rubulavirus |
Human parainfluenza virus 1, 2, 3, 4 |
Pneumoviridae |
Metapneumovirus, Orthopneumovirus |
Human metapneumovirus and Human orthopneumovirus |
Picornavirus |
Enterovirus |
Enterovirus A, B ,C ,D and Rhinovirus A, B, C |
Adenoviridae |
Mastadenovirus |
Human adenovirus B, C, E |
Parvoviridae |
Bocaparvovirus |
Primate bocaparvovirus 1 and Primate bocaparvovirus 2 |
Table 2. Refseqs with whole genome covered by NanoRV Panel v1.0-Tech Access
Fig 1. Enrichment efficiency of NanoRV Panel v1.0-Tech Access. The library was constructed from COVID-19 RNA reference (Genewell, GW-CRPM002) using NadPrep DNA Universal Library Preparation Kit (for Illumina®️) combined with NadPrep Total RNA-to-DNA Module. Custom probes targeting 7 human housekeeping genes were spiked into the NanoRV Panel v1.0-Tech Access, followed by either single or double capture sequencing. Sequencing data were analyzed with Salmon v1.2.0. Reads mapped to SARS-CoV-2 or targeted housekeeping genes were counted as on-target, while rRNAs and other transcripts as off-target.
Fig 2. The abundance of SARS-CoV-2 compared to selected housekeeping genes. The libraries constructed from COVID-19 RNA reference (Genewell, GW-CRPM002) were enriched using the NanoRV Panel v1.0-Tech Access spiked with custom probes targeting 7 human housekeeping genes. Salmon v1.2.0 was used to calculate TPM.
Fig 3. Coverage uniformity across the SARS-CoV-2 genome. The library was constructed from COVID-19 RNA reference (Genewell, GW- CRPM002) followed by targeted enrichment of SARS-CoV-2 with the NanoRV Panel v1.0-Tech Access.
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