μCaler Hybrid Capture Reagents that have been optimized for targeted enrichment of small panels and, coupled with μCaler NanoBlockers and μCaler Panel based on Nanodigmbio’s independent intellectual property rights with innovative scheme design have constituted a complete liquid hybridization capture system, thus enabling the whole process completion of capture-library preparation in same day.
The μCaler SARS-Cov-2 Panel is designed based on the Wuhan-Hu-1/2019 (MN908947) reference genome with dependence on the μCaler probe design scheme. It covers all 29.9 Kb genomic regions and can be used for precise typing, variant tracking, and identification of new variants of SARS-CoV-2.
● Simplified process for easier operation
●Complete the whole process from samples to capture-library preparation in same day
● Small panel has stable and excellent capture performance
Figure 3. Genotyping and variation graph analysis using the μCaler SARS-CoV-2 Panel on SARS-CoV-2 samples collected through oropharyngeal swabs. The whole genome sequence of SARS-CoV-2 was assembled using IRMA software, and Nextclade was used for online analysis. Genotyping and variation map analysis was performed on the variant of Wuhan-Hu-1/2019 (MN908947) as the reference genome for alignment. A. Variation map of nucleotide sequence; B. Variation map of S protein amino acid variation.
Blocking
Color of tube cap | Component |
Volume 1106102 (16 rxn) |
Volume 1106101 (96 rxn) |
|
μCaler NanoBlockers (for Illumina®) | 40 μL | 210 μL |
Color of Tube Cap |
Component |
Volume 1106212 (16 rxn) |
Volume 1106211 (96 rxn) |
|
μCaler NanoBlockers (for MGI, DI) | 40 μL | 210 μL |
Packing No. | Color of Tube Cap | Component |
Volume 1105102 (16 rxn) |
Volume 1105101 (96 rxn) |
Box 1 |
|
μHyb #1 | 840 μL | 3×1700 μL |
|
μHyb #2 | 130 μL | 790 μL | |
|
Human Cot DNA | 45 μL | 260 μL | |
Box 2 |
|
Streptavidin Beads | 480 μL | 2.9 mL |
/ |
Wash Buffer A | 2×5.3 mL | 3×21 mL | |
/ |
Wash Buffer B | 2.9 mL | 20 mL |
Capture Panel
Color of Tube Cap | Component |
Volume 1101301 (96 rxn) |
|
μCaler SARS-CoV-2 Panel | 210 μL |
Type | Product | Detail | Catalog# |
Blocker | μCaler NanoBlockers (for Illumina®), 16 rxn | 16 rxn | 1106102 |
μCaler NanoBlockers (for Illumina®), 96 rxn | 96 rxn | 1106101 | |
μCaler NanoBlockers (for MGI, DI), 16 rxn | 16 rxn | 1106212 | |
μCaler NanoBlockers (for MGI, DI), 96 rxn | 96 rxn | 1106211 | |
Hybrid Capture | μCaler Hybrid Capture Reagents, 16 rxn | 16 rxn | 1105102 |
μCaler Hybrid Capture Reagents, 96 rxn | 96 rxn | 1105101 | |
Panel | μCaler SARS-CoV-2 Panel, 96 rxn | 96 rxn | 1101301 |
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