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NanoHema Panel v2.0

NanoHema Panel v2.0

Catalog Number:
#1001722
#1001721

NanoHema Panel v2.0 targets 481 genes associated with hematologic malignancies, comprehensively covering a wide range of genetic alterations including base substitutions, insertions/deletions, amplifications, and gene fusions.

This panel consists of two components:

NanoHema Panel v2.0_DNA Tube: Covers 1.7 Mb of genomic regions, including full coding regions of 436 genes, partial intronic regions of 12 genes, and the IGH Switch regions.

NanoHema Panel v2.0_RNA Tube: Covers 146 genes, optimized for fusion gene detection.

By integrating multi-omics approaches, employing a highly targeted design, and enhancing clinical utility, NanoHema Panel v2.0 offers an efficient and cost-effective solution for precision diagnosis, treatment planning, and longitudinal management of hematologic malignancies.

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Feature

● Comprehensive Multi-type Mutation Coverage
Encompasses 481 hematologic malignancy-related genes, enabling thorough analysis of base substitutions, indels, amplifications, and gene fusions.

● Dual Workflow for Enhanced Accuracy
RNA workflow compensates for the limitations of the DNA workflow by accurately identifying fusion gene partners and validating the functional impact of fusion events. The synergistic use of both workflows ensures comprehensive and precise detection.

● Flexible Customization
Supports flexible subdivision into multiple sub-panels or the addition of target genes, allowing adaptation to diverse hematologic malignancy diagnostic needs in both research and clinical applications.


Performance

Capture Performance_NanoHema Panel v2.0_DNA

图1

Figure 1. Capture performance of NanoHema Panel v2.0_DNA applied to various types of gDNA samples. A. Mappability & On-target rate; B. Target covered; C. Average sequencing depth after deduplication; D. GC bias. Using 50 ng of various gDNA samples, pre-libraries were prepared with the NadPrep EZ DNA Library Preparation Kit coupled with the NadPrep Universal Stubby Adapter (UDI) Module, followed by hybrid capture using NanoHema Panel v2.0_DNA with NadPrep Hybrid Capture Reagents. Sequencing was performed on both NovaSeq 6000 (PE150) and DNBSEQ-T7 (PE150) platforms, with a random subset of 1.3 Gb used for data analysis.
Note: LDT-831: hematologic malignancy gDNA quality control (LDT Bioscience; LDT-831A: AF = 5%; LDT-831D: AF = 0%). Male: human genomic DNA standard (Promega, G1471).



Stable Detection of Multiple Mutation Types_NanoHema Panel v2.0_DNA

Table 1. Detection of various mutation types in different standard samples using NanoHema Panel v2.0_DNA.

图1

Capture Performance_NanoHema Panel v2.0_RNA

图1

Figure 2. Composition of capture sequencing data using NanoHema Panel v2.0_RNA. For the K562 cell line RNA (50 ng), pre-libraries were prepared using the NadPrep Total RNA-To-DNA Module coupled with the NadPrep DNA Library Preparation Kit v2, followed by hybrid capture with NanoHema Panel v2.0_RNA and NadPrep Hybrid Capture Reagents. Sequencing was performed on NovaSeq 6000 (PE150) (A) and DNBSEQ-T7 (PE150) (B), with analysis performed using Salmon.

Fusion Detection_NanoHema Panel v2.0_RNA

Table 2. Detection of fusion genes in K562 cell line RNA using NanoHema Panel v2.0_RNA.

图1

Note: Data are reported per M read pairs (approximately 0.3 Gb); analysis was performed using Arriba.

Gene List

NanoHema Panel v2.0_DNA_436 genes_Full CDS
ABL1 ABL2 ACTB AKT1 AKT2 AKT3 ALK AMER1 ANKRD26 AP3B1 APC AR ARAF ARHGAP26 ARID1A
ARID1B ARID2 ARID5B ASXL1 ASXL2 ASXL3 ATG5 ATM ATR ATRX ATXN1 AURKA AURKB AXIN1 AXL
B2M BAALC BAP1 BARD1 BCL10 BCL11A BCL11B BCL2 BCL6 BCL7A BCOR BCORL1 BIRC3 BLM BLNK
BRAF BRCA1 BRCA2 BRD4 BTG1 BTG2 BTK BTLA CALR CARD11 CBFB CBL CBLB CCND1 CCND2
CCND3 CCNE1 CCR4 CCR7 CCT6B CD22 CD27 CD274 CD28 CD58 CD70 CD79A CD79B CD83 CDC42
CDC73 CDH1 CDK12 CDK4 CDK6 CDK8 CDKN1A CDKN1B CDKN1C CDKN2A CDKN2B CDKN2C CEBPA CEP57 CHD2
CHD8 CHEK1 CHEK2 CIITA CKS1B CREBBP CRLF2 CSF1R CSF3R CTCF CTLA4 CTNNB1 CUX1 CXCR4 CYLD
DAXX DDR2 DDX3X DDX41 DHX15 DNM2 DNMT3A DOT1L DTX1 DUSP2 DUSP22 EBF1 ECT2L EED EGFR
EP300 EPCAM EPHA2 EPHA3 EPHA5 EPHA7 EPOR ERBB2 ERBB3 ERBB4 ERCC4 ERG ESR1 ETNK1 ETS1
ETV1 ETV4 ETV6 EZH2 FAM46C FANCA FANCC FANCD2 FANCE FANCF FANCG FANCI FANCJ FANCL FANCM
FAS FAT1 FAT4 FBXO11 FBXW7 FGFR1 FGFR2 FGFR3 FGFR4 FH FLCN FLT1 FLT3 FLT4 FOXO1
FOXO3 FOXP1 FYN GADD45B GATA1 GATA2 GATA3 GNA11 GNA13 GNAQ GNAS GNB1 GRIN2A HACE1 HBA1
HBA2 HBB HDAC1 HDAC4 HDAC7 HGF HIST1H1A HIST1H1C HIST1H1D HIST1H1E HNF1A HRAS ID3 IDH1 IDH2
IFNAR2 IGF1R IGH IGK IGL IGLL5 IKBKE IKZF1 IKZF2 IKZF3 IL3 IL7R INPP4B INPP5D IRF1
IRF4 IRF8 ITK ITPKB JAK1 JAK2 JAK3 JARID2 JUN KAT6A KDM2B KDM5A KDM5C KDM6A KDR
KIT KLF2 KLHL5 KLHL6 KMT2A KMT2B KMT2C KMT2D KRAS LEF1 LMO2 LRP1B LTB LYN LYST
MAF MAFB MALT1 MAP2K1 MAP2K2 MAP2K4 MAP3K1 MAP3K14 MAPK1 MCL1 MDM2 MDM4 MED12 MEF2B MEF2D
MEN1 MET MFHAS1 MGA MITF MLH1 MPL MSH2 MSH3 MSH6 MTOR MUTYH MYB MYC MYCL
MYCN MYD88 NBN NCKAP1L NCSTN NF1 NF2 NFE2L2 NFKB1 NFKB2 NFKBIA NFKBIE NKX2-1 NLRC4 NOTCH1
NOTCH2 NPM1 NRAS NSD1 NT5C2 NTRK1 NTRK3 NUP98 P2RY8 PAG1 PAK3 PALB2 PAX5 PBRM1 PC
PDCD1 PDCD1LG2 PDGFRA PDGFRB PDK1 PHF6 PIK3CA PIK3CD PIK3R1 PIK3R2 PIM1 PLCG1 PLCG2 PML PMS2
POLE POT1 POU2AF1 PPM1D PPP2R1A PRDM1 PRF1 PRKCB PRPF8 PTCH1 PTEN PTPN1 PTPN11 PTPN2 PTPN6
PTPRD PTPRK PTPRO RAB27A RAD21 RAD50 RAD51 RAF1 RARA RASGEF1A RB1 RECQL4 REL RELN RET
RHOA RICTOR RNF43 ROS1 RPL10 RPS15 RPTOR RUNX1 SAMD9 SAMD9L SBDS SDHA SDHB SDHC SDHD
SETBP1 SETD2 SF3B1 SGK1 SH2B3 SH2D1A SLC19A1 SLC7A7 SMAD2 SMAD4 SMARCA2 SMARCA4 SMARCB1 SMC1A SMC3
SMO SOCS1 SOX11 SOX2 SPEN SPOP SRC SRP72 SRSF2 STAG2 STAT3 STAT4 STAT5A STAT5B STAT6
STK11 STX11 STXBP2 SUFU SUZ12 SYK TBL1XR1 TCF3 TCL1A TERC TERT TET1 TET2 TET3 TGFBR2
TNFAIP3 TNFRSF11A TNFRSF14 TNFRSF17 TNFRSF1B TOP1 TOP2A TP53 TP63 TP73 TRAF2 TRAF3 TRAF5 TSC1 TSC2
TSHR TYK2 U2AF1 UNC13D VAV1 VHL WHSC1 WT1 XIAP XPC XPO1 ZAP70 ZBTB7A ZEB2 ZMYM3
ZRSR2 　 　 　 　 　 　 　 　 　 　 　 　 　 　
NanoHema Panel v2.0_DNA_12 genes_Selected Intron
ALK BCL6 BCR BRAF ETV6 JAK2 KMT2A MYC PDGFRA PDGFRB RARA RET
NanoHema Panel v2.0_RNA_146 genes
ABL1 ABL2 ALK ARHGAP26 ARID1A ASXL1 ATG5 BCL10 BCL11A BCL11B BCL2 BCL3 BCL6 BCL7A BCL9
BCOR BCR BIRC3 BRAF BTG1 CBFA2T3 CBFB CBL CCND1 CCND2 CCND3 CD274 CDK6 CEBPE CIITA
CREBBP CRLF2 CTNNB1 DEK DUSP22 DUX4 EGFR EP300 EPOR ERBB2 ERG ETS1 ETV1 ETV4 ETV6
EWSR1 FGFR1 FGFR2 FGFR3 FOXO1 FOXO3 FOXP1 GLIS2 HLF IGH IGK IGL IKZF1 IL3 IRF4
ITK JAK1 JAK2 JAK3 KAT6A KIF5B KMT2A LMO1 LMO2 LYN MAF MAFB MALT1 MECOM MEF2D
MLLT1 MLLT10 MLLT3 MN1 MTCP1 MYB MYC MYH11 NCOA2 NCOR1 NF1 NF2 NFKB2 NOTCH1 NPM1
NSD1 NTRK1 NTRK3 NUP214 NUP98 NUTM1 P2RY8 PAX5 PBX1 PDCD1LG2 PDGFRA PDGFRB PICALM PIM1 PML
POU2AF1 PRDM1 PTCH1 RAF1 RARA RARB RARG RBM15 RET ROS1 RUNX1 RUNX1T1 STAT6 STIL SYK
TAF15 TAL1 TBL1XR1 TCF3 TCL1A TCL1B TET1 TLX1 TLX3 TNFRSF11A TOP1 TP63 TPM3 TPM4 TRA
TRB TRD TRG TYK2 VAV1 WHSC1 ZBTB16 ZEB2 ZNF292 ZNF362 ZNF384 　 　 　 　

#Package Cap Color Component Volume
1001721 (96 rxn) 		Volume
1001722 (16 rxn)
Box 1

NanoHema Panel v2.0_DNA 415 μL 70 μL

NanoHema Panel v2.0_RNA 415 μL 70 μL
Why integrate DNA and RNA workflows?

Fusion genes serve as critical molecular markers for the diagnosis and treatment of hematologic malignancies, making their accurate detection essential. The DNA workflow detects gene fusions by designing probes covering intronic regions; however, due to the large size and high repetitiveness of introns, relying solely on DNA detection poses challenges such as high cost and incomplete coverage, and it cannot confirm the functional impact of fusion events. In contrast, the RNA workflow directly focuses on the transcript level, enabling precise identification of actual fusion events and their partner genes while assessing whether these fusions lead to aberrant transcription. Consequently, the combined use of DNA and RNA workflows provides a “dual safeguard” for fusion gene detection, enhancing both the comprehensiveness and accuracy of the results.

Advantages of RNA capture sequencing over direct total RNA-Seq:

Total RNA-seq requires rRNA depletion and individual reactions per sample, increasing both complexity and cost. Moreover, total RNA-seq exhibits relatively lower sensitivity, particularly in detecting low-frequency fusion events in low-expression samples. In contrast, the DNA panel in NanoHema Panel v2.0 already covers the intronic regions of key genes, allowing users to flexibly add custom intronic regions based on requirements and budget without the need for a separate RNA panel.

Does this panel support personalized customization?

NanoHema Panel v2.0 is a comprehensive large panel that covers a wide range of hematologic malignancy-associated gene variants. To meet diverse clinical needs, the panel offers multiple customization options:

•  It can be flexibly split into multiple sub-panels (e.g., for acute myeloid leukemia [AML], lymphoblastic leukemia, T-cell lymphoma, and B-cell lymphoma).

•  Additional target genes can be incorporated based on specific research or diagnostic requirements, enabling the creation of a personalized detection solution.

Product Catalog#
NanoHema Panel v2.0, 96 rxn 1001721
NanoHema Panel v2.0, 16 rxn 1001722

Product Sheet

Application Note

Demo Data

NanoHema_Panel_v2.0_DNA_LDT-831A_Illumina
For the hematologic malignancy gDNA quality control (LDT Bioscience, LDT-831A), pre-libraries were prepared using the NadPrep EZ DNA Library Preparation Kit, followed by hybrid capture with NanoHema Panel v2.0_DNA and NadPrep Hybrid Capture Reagents.
Download
NanoHema_Panel_v2.0_DNA_LDT-831D_Illumina
For the hematologic malignancy gDNA quality control (LDT Bioscience, LDT-831D), pre-libraries were prepared using the NadPrep EZ DNA Library Preparation Kit, followed by hybrid capture with NanoHema Panel v2.0_DNA and NadPrep Hybrid Capture Reagents.
Download
NanoHema_Panel_v2.0_DNA_G1471_Illumina
For the human genomic DNA standard (Promega, G1471), pre-libraries were prepared using the NadPrep EZ DNA Library Preparation Kit, followed by hybrid capture with NanoHema Panel v2.0_DNA and NadPrep Hybrid Capture Reagents.
Download
NanoHema_Panel_v2.0_RNA_K562_Illumina
For the K562 cell line RNA, pre-libraries were prepared using the NadPrep Total RNA-To-DNA Module coupled with the NadPrep DNA Library Preparation Kit v2, followed by hybrid capture with NanoHema Panel v2.0_RNA and NadPrep Hybrid Capture Reagents.
Download
NanoHema_Panel_v2.0_DNA_LDT-831A_MGI
For the hematologic malignancy gDNA quality control (LDT Bioscience, LDT-831A), pre-libraries were prepared using the NadPrep EZ DNA Library Preparation Kit, followed by hybrid capture with NanoHema Panel v2.0_DNA and NadPrep Hybrid Capture Reagents.
Download
NanoHema_Panel_v2.0_DNA_LDT-831D_MGI
For the hematologic malignancy gDNA quality control (LDT Bioscience, LDT-831D), pre-libraries were prepared using the NadPrep EZ DNA Library Preparation Kit, followed by hybrid capture with NanoHema Panel v2.0_DNA and NadPrep Hybrid Capture Reagents.
Download
NanoHema_Panel_v2.0_DNA_G1471_MGI
For the human genomic DNA standard (Promega, G1471), pre-libraries were prepared using the NadPrep EZ DNA Library Preparation Kit, followed by hybrid capture with NanoHema Panel v2.0_DNA and NadPrep Hybrid Capture Reagents.
Download
NanoHema_Panel_v2.0_RNA_K562_MGI
For the K562 cell line RNA, pre-libraries were prepared using the NadPrep Total RNA-To-DNA Module coupled with the NadPrep DNA Library Preparation Kit v2, followed by hybrid capture with NanoHema Panel v2.0_RNA and NadPrep Hybrid Capture Reagents.
Download
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