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What’s the difference between non-stranded and stranded libraries from NadPrep EZ RNA Library Preparation Kit?

View: 63 / Time: 2025-11-04
RNA-Seq library preparation generally falls into two types: non-stranded (general) and stranded (strand-specific). The core difference is whether the library retains transcript directional information. Among them, stranded libraries retain the transcript directional information during the preparing process, and sequencing and downstream analysis can determine whether the transcripts originate from the sense or antisense strands of DNA. However, non-stranded libraries lose directional information during cDNA synthesis, and sequencing data cannot directly indicate transcript directions. Compared with general RNA-Seq, strand-specific RNA-Seq provides more accurate transcript quantification, clearer gene structures, identification of antisense transcripts, and discovery of novel transcripts. Therefore, stranded RNA-Seq is crucial for studies of gene structure and expression regulation. To obtain transcript direction information, stranded libraries should be preferred.


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