What can cause low library yield when using the NadPrep EZ RNA Library Preparation Kit? How to solve it?

View: 87 / Time: 2025-11-05

RNase contamination: Use RNase-free consumables throughout the workflow and perform all the operations in RNase-free conditions. RNase contamination will reduce reverse transcription efficiency and low library yield.
Low sample purity: Residual contaminants in RNA (e.g. chelators, guanidine salts, phenol, ethanol) may inhibit the activity of reverse transcriptase. It is recommended to purify RNA according to Step 7:Post-amplification Cleanup using 2X NadPrep SP Beads and elute in Nuclease Free Water.
Poor sample quality: For low-quality RNA samples, follow the recommendations to increase the no. of PCR cycles appropriately.

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