Is it appropriate to use input DNA that is higher or lower than recommended in the user manual?

In order to ensure the data quality, it is best to prepare the library within the scope recommended in the user manual. If there are special experimental needs, it can also be adjusted appropriately. The libraries prepared fromdifferent input DNA areshown as follows:

Experimental conditions: Human Genomic DNA Male (Promega, catalog # G1471) was fragmented with different input DNA, with 25 min of the enzymatic digestion time. NadPrep Universal Stubby Adapter (UDI) Module was used together for library preparation with single-sided selection mode. The library yield and size meet the requirements of sequencing.